|
KMID : 0380020130280030151
|
|
Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 3 p.151 ~ p.156
|
|
|
Production and Characterization of β-Glucan Type Oligomer Produced with Enzymatic Hydrolysis of Capsosiphon fulvescens
|
|
Kim Hyun-Woo
Lee Jung-Heon
|
|
|
Abstract
|
|
|
|
-Glucan type oligomers which have angiotensin I converting enzyme (ACE) inhibitory activity were isolated and characterized from Capsosiphon fulvescens. After C. fulvescens was hydrolysis with Alcalase at 50oC, supernatant was harvested and separated with ultrafiltration membrane (MWCO 2 kDa). Oligomers which were less than 2 kDa of molecular weight were harvested for characterization. The nutrient composition of Alcalase hydrolysate was 89.9% carbohydrate, 4.2% protein and 5.9% sulfate. After ultrafiltration, the nutrient composition of oligomers was changed to 99.88% carbohydrate, 0.07% protein and 0.05% sulfate. The carbohydrate composition of oligomer was glucose (97.2%) and mannose (1.5%). The ACE inhibitory activities of Alcalase hydrolysate and oligomer were 72.1% and 82%, respectively. The molecular weight of oligomer was about 1 kDa. The oligomer was analyzed with FT-IR, 1H-NMR and methylation. The oligomers were β-1,3-glucans with β-(1,3)-linked glucose units.
|
|
|
KEYWORD
|
|
|
Capsosiphon fulvescens, β-glucan, Hypertension, Oligomers
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|